![]() The researchers found that cells proliferated far better under flow than in conditions with no fluid flow. This makes simulations much more realistic than growing cells in a flat petri dish, Mikos said. Three layers of each were stacked to make each 3-D scaffold, and these were seeded with tumor cells and placed in a flow perfusion reactor that mimics the push and pull of fluids and tissues in a biological environment. He noted that by varying the scaffold architecture, they can change the mechanical environment through which fluids flow and the magnitude of shear stress exerted on tumor cells.įlat sections of scaffold were printed with pores in one of three sizes: 0.2, 0.6 and 1 millimeter. "We aim to develop tumor models that can capture the complexity of tumors in vitro and can be used for drug testing, thus providing a platform for drug development while reducing the associated cost," Mikos said. The bone-like printed polymer contains pores of varying sizes to constrain fluids that flow through and apply varying degrees of shear stress to the tumor cells, depending on the scaffold's orientation in relation to the flow. The scaffold itself is special, according to Mikos. The research is detailed in the American Chemical Society journal ACS Biomaterials Science and Engineering. The scientists said 3-D printing allows them to get closer than ever to mimicking the architecture of real bone. The size and shape of pores and scaffold porosity-the percent of empty space in a structure created by pores-can impact cell attachment, alter the permeability of media and nutrients and facilitate cell migration, according to the researchers. The researchers determined the structure of a scaffold, natural or not, has a very real effect on how cells express signaling proteins that help cancer grow. ![]() Testing treatments for bone cancer tumors may get easier with new enhancements to sophisticated support structures that mimic their biological environment, according to Rice University scientists.Ī team led by Rice bioengineer Antonios Mikos has enhanced its three-dimensional printed scaffold to see how Ewing's sarcoma (bone cancer) cells respond to stimuli, especially shear stress, the force experienced by tumors as viscous fluid such as blood flows through bone.
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